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Generation and quantification of GSH, GSSH, persulfides and polysulfides from reaction of GSSG and Na 2 S GSSG (100 M) in 500 L of 50 mM HEPES buffer (pH 7.4) was reacted with H 2 S (100300 M) for 20 min at RT in 500 L gas tight tubes

In this case, do not add Coenzyme working solution or Enzyme working solution, Buffer solution and pure water are added